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Impact of hybridization on the pattern of molecular genetic variation in maize (Zea mays)

Ayoob O Mohammed

College of Agriculture, University of Anbar- Abu Ghraib- Baghdad/ IRAQ

Corresponding Author Email: dr.job77@gmail.com (Ayoob O Mohammed)

Page No: 479 - 486

Keywords: Maize, Hybridization, Genetic Polymorphism and RAPD marker

Received - September 24, 2015; Revision - October 05, 2015; Accepted - November 08, 2015 Available Online - December 10, 2015

doi: http://dx.doi.org/10.18006/2015.3(6).479.486

Abstract

Present study was conducted to analyze the impact of hybridization on the molecular variation of maize. Total ten RAPD markers were used to assess the genetic diversity in hybridized maize crop and its relationships with their respective F1 hybrids was also established in present study. Among these, five were succeeding to amplify DNA fragments through PCR technique and produced total 31 fragments. Out of these thirty one, twenty two showed polymorphism and mean of these polymorphic percent scored 64.5%. Least genetic similarity was reported in L2×T1 against other genotypes; on the other hand L2×T2 and L3×T1 became closely related genera and gained 100 percent genetic similarity. Generally, the F1 hybrids acted in a different way compared with their corresponded parental lines, and the latest tend to occupy separate cluster in Dendrogram tree as they generally gained low values of genetic similarity. Meanwhile F1 hybrids showed high level genetic similarity among themselves. Result of present study revealed a clear impact of hybridization on the genetic diversity among maize genotypes and could play an important role in developing new genotypes.

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