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Identification and purification of novel chlorogenic acids in Artemisia annua L.

Wenwen Zhao1, Weina Zhang1, Yuru Chen1,*, Fengping Yang1, Qiming Cao2, Wenzhong Chen3, Junli Liu4 and Kewei Dai5

1College of Life Science, Nanjing Normal University, Nanjing Engineering Research Center for Industrialization of Microbial Resources, Jiangsu Key Lab for Biodiversity and Biotechnology, Jiangsu Key Lab for Microbes and Functional Genomics. Nanjing 210023, P. R. China
2School of Resources and Environment, University of Electronic Science and Technology of China, Chengdu 610054, P.R. China
3Nanjing Luye Sike Pharmaceutical Co., Ltd. Nanjing, 210061, P. R. China
4Institute of Chemical Industry of Forest Products, CAF, Nanjing 210042, P. R. China
5College of Geography Science, Nanjing Normal University, Nanjing 210023, P. R. China

Corresponding Author Email: chenyuru@njnu.edu.cn (Yuru Chen)

Page No: 415 - 422

Keywords: Artemisia annua L., Chlorogenic acids, Dicaffeoylquinic acids, LC/MSn, Identification and Purification

Received - August 28, 2015; Revision - September 14, 2015; Accepted - October 04, 2015 Available Online - October 20, 2015

doi: http://dx.doi.org/10.18006/2015.3(5).415.422

Abstract

Present work has been carried out to study the identification and purification of chlorogenic acids in Artemisia annua L. Thirty-six chlorogenic acids were identified from this plant. Among these fifteen viz. two monocaffeoylquinic acids (Mr354), five dicaffeoylquinic acids (Mr516), one feruloylquinic acid (Mr368), three caffeoylferuloylquinic acids (Mr530), two ferulylquinic acids (Mr544), one dimethoxy-cinnamoylquinic acid (Mr382) and one p-coumaroylquinic acid (Mr338) were reported first time in present study by LC/MSn. Cis-isomers of these chlorogenic acids were also identified. Furthermore, column chromatography was used for the separation and purification of these chlorogenic acid; by the use of petroleum ether and ethyl acetate decolorization methods as mentioned in the literature, thus separation and purification process carried out at the same time. Polyamide and dextran were also used to purify Dicaffeoylquinic acid and purity level reached 85.7% with a yield of 53.4% after the secondary purification by Sephadex LH-20. Result of study revealed that A. annua can not only used for the production of artemisinin, but also yielding different kinds of chlorogenic acids, thus making comprehensive utilization of this plant.

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