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Impact of geographic’s variation on the essential oil yield and chemical composition of three Eucalyptus species acclimated in Tunisia

Elaissi Ameur1*, Medini Hanene1, Rouis Zied2, Khouja Mohamed Larbi3, Chemli Rachid1 and Harzallah-Skhiri Fethia1

1Laboratory of The Chemical, Galenic and pharmacological Drug Development, Faculty of Pharmacy, University of Monastir, Avenue Avicenne, 5019 Monastir, Tunisia
2Laboratory of Genetic, Biodiversity and Bio-resources Valorisation, Higher Institute of Biotechnology of Monastir, University of Monastir, Avenue Tahar Haddad, 5000 Monastir, Tunisia
3National Institute for Research on Rural Engineering, Water and Forestry, Institution of Agricultural Research and Higher Education, BP. N.2, 2080 Ariana, Tunisia

Corresponding Author Email: aelaissi@yahoo.fr (Elaissi Ameur)

Page No: 324 - 336

Keywords: Eucalyptus, Essential oils, 1,8-cineole, ACP, HCA, GC-MS, Chemotype and Variation

Received - March 28, 2015; Revision - April 10, 2015; Accepted - June 15, 2015 Available Online - July 07, 2015

doi: http://dx.doi.org/10.18006/2015.3(3).324.336


Present study has been carried out to estimate the impact of geographical distribution on the yield and chemical constitute of three Eucalyptus verities viz  E. cinerea F. Muell. ex Benth., E. astringens Maiden and  E. sideroxylon A.Cunn. ex Schauer-. These species were collected from six arboreta of Tunisia in January 2008. The essential oil was extracted by hydrodistillation method and estimated the essential oil yield which varies from 1.5±0.1% to 4.0±0.2%. Results of the study revealed that yield of essential oil are not only depends on the Eucalyptus species but also depends on the origin of harvest. E. sideroxylon A. Cunn. exWoolls, cultivated in jbel abderrahman arboreta and E. cinerea F. Muell. ex Benth. from choucha (sejnanae) arboreta provided  the lowest and the highest percentage of essential oil amongst all the studied provenances, respectively. GC (RI) and GC/MS analysis showed the presence of 163 components, representing 98.8 to 99.5% of the total oil. The contents of the different samples varied according to the species and the origin of harvest. The main components of the Eucalyptus essential oil were 1,8-cineole (39.1±0.0 – 79.4±0.0%),  followed  by α-pinene (2.1±0.0- 30.0±0.0), trans-pinocaveol  (0.7±0.5-10.0±2.5%), α -Terpineol (0.2±0.0 – 8.8±1.1%), globulol (0.3±0.0 – 5.7±1.3), aromadendrene (0.2±0.0 – 4.9±2.1%), limonene (0.6±0.0 – 4.3±0.8%), pinocarvone (0.2±0.0- 3.7±0.6%),  bicyclogermacrene (0.0 – 3.1±0.0%), α-terpinyl acetate (0.0-2.9±0.8%),  p-cymene (0.7±0.0- 2.7±2.1%), α -carophyllene  (0.3±0.0-1.9±0.0%), viridiflorol (0.2±0.0- 1.8±0.%), epiglobulol (traces – 1.3±0.6%) and spathulenol (0.2±0.0- 1.1±0.0%). The principal component and the hierarchical cluster analyses separated the Eucalyptus populations leaf essential oils into four groups, each constituting a chemotype. E. sideroxylon from Korbous and Sejnane arboreta afforded the highest mean percentage in 1,8-cineole.

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