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A simple and efficient dye-based technique for rapid screening of fungi for L-asparaginase production

Anil kumar Meghavarnam and Savitha Janakiraman*

Department of Microbiology and Biotechnology, Bangalore University, Jnanabharathi campus, Bangalore- 560056, India

Corresponding Author Email: drsvtj@yahoo.co.in (Savitha Janakiraman)

Page No: 123 - 130

Keywords: L-asparaginase, Indicator dye, Bromo cresol purple, Phenol red and Fungi

Received - January 30, 2015; Revision - February 07, 2015; Accepted - March 11, 2015 Available Online April 25, 2015

doi:http://dx.doi.org/10.18006/2015.3(2).123.130

Abstract

Three hundred and sixty four (364) isolates of tropical soil fungi were screened for L-asparaginase production by rapid plate method using modified Czapek-Dox agar containing L-asparagine, and either bromo cresol purple or phenol red dye as an indicator. Results of the study revealed that total one hundred and thirty five (135) isolates showed positive reaction for L-asparaginase production as indicated by the color change in and around the colonies between 48-72 hours of incubation at 280C. A comparative study of the two indicator dyes with varying concentrations showed bromo cresol purple is a better and efficient indicator for L-asparaginase screening than phenol red. Quantitative estimation of L-asparaginase in the selected fungi showed Aspergillus sp. and Fusarium sp. were good candidates for L-asparaginase production.

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