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Effect of moisture levels on the growth, nodulation and nitrogen fixation in Dalbergia sissoo by Azospirillum brasilense and Acaulospora laevis

Gaurav Bhushan1,*, Santosh Kr Sharma2, Saurabh Kumar1, Sweta Chhangani2, Jaspal Singh1 and Singh A P1

1Department of Environmental science, Bareilly College, Bareilly (U.P.) India
2Department of Botany, University of Rajasthan, Jaipur (Raj.) India

Corresponding Author's Email: bhushang25@gmail.com (Gaurav Bhushan)

Page No: 432 438

Keywords: Dalbergia sisso, Azospirillum brasilense, Acaulospora laevis, Moisture stress, Nodulation and N2 fixation

Received May 18, 2014; Revision June 06, 2014, Accepted August 20, 2014 Available Online August 27, 2014

Abstract

In present study, influence of single and combined inoculation of Azospirillum brasilense and Acaulospora laevis on the growth, nodulation and N2 fixation in Dalbergia sissoo was tested at various moisture levels under pot culture conditions. Combined inoculation of A.brasilense and A.laevis was found best in raising maximum growth, nodulation, nitrogen fixation and percentage of AM colonization in roots. These characters were considerably influenced with the increasing moisture stress from -0.3MPa to -1.5MPa. Furthermore, the values of all the above mentioned parameters show decrement with the increasing of moisture stress. Plants with dual inoculation performed better than single inoculated plants. Observation of the present study counted a protective role played by AM in providing resistance to D.sissoo against injurious effects of moisture stress.

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