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The selection and content determination of an anti-migraine effective fraction separated from TianMa GouTeng Decoction by macroporous resin

Zhaohuan Lou1, Bohou Xia1, Junyan Zhao2, Yuefang Huang3, Mingjie Hu1 and Guangji Zhang ,*

1Institute of Material Medica, Zhejiang Chinese Medical University, 548 Binwen Road, Hangzhou, 310053, China
2The First People Hospital of Hangzhou Xiaoshan District, 199 Shixin South Road, Xiaoshan District, Hangzhou, 311200, China
3Department of Neurology ,Zhejiang Provincial Hospital of TCM, 54 Youdian Road,Hangzhou, 310006, China

Corresponding Author's Email: loutcm@gmail.com (Guangji Zhang)

Page No: 364 -372

Keywords: Migraine, Jasminoidin, Baicalin, Traditional Chinese medicine and RP-HPLC

Received June 18, 2014; Revision July 08, 2014, Accepted August 09, 2014 Available Online August 21, 2014

Abstract

The objective of the present study was to obtain an effective part of Tianma Gouteng decoction (TMGTD) on anti-migraine and to establish a RP-HPLC method for the content determination of Jasminoidin and Baicalin in the part. For this study, TMGTD extract was prepared by decoction with water and 30%, 60% and 90% part of TMGTD were made by D-101 macroporous resin. Migraine rat model induced by subcutaneous injection of Nitroglycerine was applied to evaluate the anti-migraine effect of the samples. RP-HPLC coupled with liquid-solid extraction method was applied for the content determination of Jasminoidin and Baicalin. Results: 60% fraction was the most effective part of TMGTD on anti-migraine, with significant effect on reducing the frequency of head scratching and regulating the abnormal content of 5-HT, DA, NE and E in serum and brain tissue of migraine rats. The calibration curve of Jasminoidin and Baicalin was linear (r>0.999) over the range of 0.0153 to 0.183 g and 0.334 to 4.013 g respectively, and the average recovery of each component was from 96.1% to 100.1% and 97.6% to 102.7% respectively. The content of Jasminoidin and Baicalin in 60% fraction was 1.03% and 29.6%, which was significantly higher than that in the fraction before purification process optimization. Conclusions: This was the first report on anti-migraine effective part selection and main components content determination of TMGTD. The result may provide a meaningful basis for the clinical application of TMGTD on anti migraine headache and for the new product development of TMGTD effective part.

Journal of Experimental Biology and Agricultural Sciences COPYRIGHT 2014 ALL RIGHTS RESERVED